Caspase-1 (human), (recombinant)

Product Specifications

Specifications

One U=1 pmol/min, using Ac-YVAD-pNA (200μM; Prod. No. ALX-260-026) as substrate, at 30°C.

Product Name Alternative

Interleukin 1β converting enzyme, IL-1β converting enzyme, ICE

Certification

RUO

UniProt

P29466

Source

Produced in E. coli . cDNA encodes residues identical to Asn 120 -His 404 (C-terminus) at Genbank Accession No. M87507, except for an Asp 381 to Glu change, introduced to stabilize the enzyme against autoproteolysis.

Purification

Purified by multi-step chromatography.

Stability

After initial defrost, aliquot product into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, undiluted and unused enzyme should be refrozen quickly by, for example, snap-freezing in a dry ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles.