Calcineurin cellular activity assay kit

Store all components except the microtiter plate at 80°C for the highest stability. The calcineurin enzyme component SE163-9090 must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be quickly refrozen by placing at -80°C. To minimize the number of freeze/thaw cycles, aliquot the calcineurin into separate tubes and store at -80°C.

Calcineurin enzyme, Calmodulin (human, recombinant), Substrate (RII phosphopeptide), 2x assay buffer, EGTA Buffer, Lysis Buffer, Protease inhibitor cocktail, Biomol GreenTM Reagent, Phosphate standard, Okadaic acid, Desalting column and resin, 1⁄2-Volume microplate