BIOARRAYTM Low Input RNA Amplification and Biotin Labeling System

This product is manufactured and sold by ENZO LIFE SCIENCES, INC. for research use only by the end-user in the research market and is not intended for diagnostic or therapeutic use. Purchase does not include any right or license to use, develop or otherwise exploit this product commercially. Any commercial use, development or exploitation of this product or development using this product without the express prior written authorization of ENZO LIFE SCIENCES, INC. is strictly prohibited. Enzo and BIOARRAY are trademarks of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

The BIOARRAYTM Low Input RNA Amplification with Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (a RNA) from total cellular RNA samples in less than 24 hours for gene expression analysis.

A RNA yield must ≥ 20μg a RNA purity must range between an A 260 /A 280 ratio of 1.9 and 2.3 a Median aRNA size must be equal to or greater than 1200 nt Affymetrix GeneChip criteria: % Present calls ≥ 40% Scale factors ≤ 3.0 3’/5′ ratios for actin and GAPDH ~ 3.0 and 1.5 respectively

40 μl, First Strand Buffer (FSB) 10 μl, First Strand Enzyme Mix (FSE) 30 μl, Second Strand Buffer Mix (SSB) 20 μl, Second Strand Enzyme Mix (SSE) 250 μL, Transcription Buffer Mix (TB) 75 μl, DTT (DTT) 75 μl, Enhancer Cocktail (EC) 50 μL, T7 RNA Polymerase (T7) 1 ml, Nuclease-free Water (W)