BIOARRAY HIGHYIELD®RNA Transcript Labeling Kit (T7)

Yield: Each lot is shown to generate 50 μg of cRNA when transcribed from a double stranded cDNA library generated from 2 μg total RNA (mixed tissue) in a single round of amplification. Incorporation: Each lot is tested to ensure at least 10 % of incorporated nucleotides are biotin labeled. RNase activity: Insignificant amounts of RNase (less than 100 fg RNase A activity in 1 μL of reagent) are detected. % P value and 3’/5’ ratio: The cRNA synthesized by the kit is tested on Affymetrix HU133A 2.0 chip and shown to give > 35% P-calls, with 3’/5’ ratios for both β-actin and GAPDH of < 2.5.