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BAFF (soluble) (mouse), (recombinant)

Product Specifications

Product Name Alternative

BLyS, TALL-1, THANK, zTNF4, TNFSF 13B/20, CD257

Certification

RUO

Gene ID

A000383 (UCSD Signaling Gateway ID)

UniProt

Q9WU72

Source

Produced in HEK 293 cells. The extracellular domain of mouse BAFF (aa 127-309) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or other carrier protein.

Molecular Weight

~17kDa (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that BAFF mediates splenocyte survival. FLAG is a registered trademark of Sigma-Aldrich Co.

Specificity

Binds to human (weak) and mouse BCMA, TACI and BAFF-R.

Formulation

Lyophilized. Contains PBS.

Applications Notes

ELISA: binds to BAFF receptor at 1-100 ng/ml.

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