BAFF (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
THANK, zTNF4, TALL-1, BlyS, TNFSF 13B/20, CD257
Certification
RUO
UniProt
Q9Y275
Source
Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<1EU/μg purified protein (LAL test; Associates of CAPE COD Inc.) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~28kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that BAFF mediates splenocyte survival and induces monocyte survival. FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse (weak) BCMA, TACI and BAFF-R.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to BAFF receptor at 2-100 ng/ml. Detection of receptors of human soluble BAFF on target cells.
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