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BAFF (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

THANK, zTNF4, TALL-1, BlyS, TNFSF 13B/20, CD257

Certification

RUO

UniProt

Q9Y275

Source

Produced in E. coli. The extracellular domain of human BAFF (aa 83-285) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<1EU/μg purified protein (LAL test; Associates of CAPE COD Inc.) .

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Molecular Weight

~28kDa (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that BAFF mediates splenocyte survival and induces monocyte survival. FLAG is a registered trademark of Sigma-Aldrich Co.

Specificity

Binds to human and mouse (weak) BCMA, TACI and BAFF-R.

Formulation

Lyophilized. Contains PBS.

Applications Notes

ELISA: binds to BAFF receptor at 2-100 ng/ml. Detection of receptors of human soluble BAFF on target cells.

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