BACE2 (soluble) (human), (recombinant) (His-tag)
Product Specifications
Specifications
≥20 U/μg enzyme. One unit will hydrolyze one pmole Mca-KPLGL-Dpa-AR substrate (Prod. No. BML-P276) (10μM) per minute at 37°C, in 50mM NaOAc, pH 4.5, 1M sodium chloride.
Product Name Alternative
β-Secretase 2, β-Site APP-cleaving enzyme 2, Memapsin-1, ASP-1
Certification
RUO
UniProt
Q9Y5Z0
Source
Produced in insect cells. Recombinant soluble BACE2, cloned from human cDNA, secreted as zymogen from insect cells, purified using a C-terminal His-tag, and cleaved to the mature active form. Produced in a baculovirus expression system.
Sequence
Aa Ala 21 -Pro 466 .
Purification
Purified by multi-step chromatography.
Stability
BACE2 is stable after 5 freeze-thaws at 0.3-0.4 μg/μl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.
Purity
≥80% (SDS-PAGE)
Molecular Weight
~45kDa (calculated) ; ~48kDa (doublet by SDS-PAGE)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 20% glycerol.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates, and screen for BACE2 inhibitors or BACE1 inhibitor selectivity.
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