BACE2 (soluble) (human), (recombinant) (His-tag)
Product Specifications
Specifications
≥20 U/μg enzyme. One unit will hydrolyze one pmole Mca-KPLGL-Dpa-AR substrate (Prod. No. BML-P276) (10μM) per minute at 37°C, in 50mM NaOAc, pH 4.5, 1M sodium chloride.
Product Name Alternative
β-Secretase 2, β-Site APP-cleaving enzyme 2, Memapsin-1, ASP-1
Certification
RUO
UniProt
Q9Y5Z0
Source
Produced in insect cells. Recombinant soluble BACE2, cloned from human cDNA, secreted as zymogen from insect cells, purified using a C-terminal His-tag, and cleaved to the mature active form. Produced in a baculovirus expression system.
Sequence
Aa Ala 21 -Pro 466 .
Purification
Purified by multi-step chromatography.
Stability
BACE2 is stable after 5 freeze-thaws at 0.3-0.4 μg/μl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.
Purity
≥80% (SDS-PAGE)
Molecular Weight
~45kDa (calculated) ; ~48kDa (doublet by SDS-PAGE)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 20% glycerol.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates, and screen for BACE2 inhibitors or BACE1 inhibitor selectivity.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS7207281-01 48 Well
Rabbit Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit
Sign In for Pricing
View Details MBS7207281-02 96 Well
Rabbit Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit
Sign In for Pricing
View Details MBS7207281-03 5x 96 Well
Rabbit Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit
Sign In for Pricing
View Details MBS7207281-04 10x 96 Well
Rabbit Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit
Sign In for Pricing
View Details