AMPINEXTTM RNA Bisulfite-Seq Kit (Illumina)

Upon receipt: (1) Store the following components at -20°C immediately: 5X RT Reaction Buffer, 10 mM dNTP mix, 0.1M DTT, RNase Inhibitor, Random Primer, RT Enzyme Mix, 10X End Polishing Buffer, End Polishing Enzyme Mix, End Polishing Enhancer, 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature.

A complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a “post-bisulfite” library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.

Conversion Buffer Conversion Powder NA Binding Solution F-Spin Column F-Collection Tube Desulphonation Solution 5X RT Reaction Buffer 10 mM dNTP Mix 0.1M DTT RNase Inhibitor Random Primer (50 μM) RT Enzyme Mix 10X End Polishing Buffer End Polishing Enzyme Mix End Polishing Enhancer 2X Ligation Buffer T4 DNA Ligase Adaptors (50 μM) MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 μM) Primer I (10 μM) Elution Buffer