AMPINEXTTM Post-Bisulfite DNA Library Preparation Kit (Illumina)

Upon receipt, store the following components at -20°C immediately: 5X Conversion Buffer, Conversion Enzyme Mix, Conversion Primer, 10X End Repair Buffer, End Repair Enzyme Mix,10X dA-Tailing Buffer, Klenow Fragment (3’-5’ exo-), 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature. ​

A complete set of optimized reagents to prepare a DNA library, after successful bisulfite conversion, for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays.

5X Conversion Buffer Conversion Enzyme Mix Conversion Primer 10X End Repair Buffer End Repair Enzyme Mix 10X dA-Tailing Buffer Klenow Fragment (3’-5’ exo –) 2X Ligation Buffer T4 DNA Ligase Adaptors (50 μM) MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 μM) Primer I (10 μM) Elution Buffer