AMPINEXTTM High-Sensitivity DNA Library Preparation Kit (Illumina)

Upon receipt: Store the following components at -20°C immediately: 10X End Polishing Buffer, End Polishing Enzyme Mix, End Polishing Enhancer, 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature. ​

Starting materials can include fragmented dsDNA isolated from various tissue or cell samples, dsDNA enriched from a ChIP reaction, MeDIP/hMeDIP reaction, or exon capture. DNA should be relatively free of RNA because large fractions of RNA will impair end repair and dA-tailing, resulting in reduced ligation capabilities. The input amount of DNA can be from 0.2 ng to 100 ng. For optimal preparation, the input amount should be 10 ng to 50 ng.

10X End Polishing Buffer End Polishing Enzyme Mix End Polishing Enhancer 2X Ligation Buffer T4 DNA Ligase Adaptors (50 μM) MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 μM) Primer I (10 μM) Elution Buffer