AMPINEXTTM DNA Library Preparation Kit (Illumina)

Upon receipt: Store the following components at -20°C immediately: 10X End Repair Buffer, End Repair Enzyme Mix,10X dA-Tailing Buffer, Klenow Fragment (3’-5’ exo-), 2X Ligation Buffer, T4 DNA Ligase, Adaptors, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. Store the following components at 4°C: MQ Binding Beads. Store all other components at room temperature. ​

Starting materials can include fragmented dsDNA isolated from various tissue or cell samples, dsDNA enriched from ChIP reactions, MeDIP/hMeDIP reaction, or exon capture. DNA should be relatively free of RNA since large fractions of RNA will impair end repair and dA tailing, resulting in reduced ligation capabilities. Input amount of DNA can be from 5 ng to 1 μg. For optimal preparation, the input amount should be 100 ng to 200 ng. For amplification-free, 500 ng or more is needed.

10X End Repair Buffer End Repair Enzyme Mix 10X dA-Tailing Buffer Klenow Fragment (3’-5’ exo –) 2X Ligation Buffer T4 DNA Ligase Adaptors (50 μM) MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 μM) Primer I (10 μM) Elution Buffer