AMPINEXTTM 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

Upon receipt: (1) Store the following components at -20°C immediately: 5X Reaction Buffer, Reaction Enzyme Mix, Adaptor-A, Adaptor-B, RNA Digestion Buffer, RNA Digestion Enzyme, 2X HiFi PCR Master Mix, Primer U, Primer I, and Elution Buffer. (2) Store the following components at 4°C: MQ Binding Beads. (3) Store all other components at room temperature away from light.

A complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a “post-bisulfite” library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.

Conversion Buffer Conversion Powder NA Binding Solution F-Spin Column F-Collection Tube Desulphonation Solution 5X Reaction Buffer Reaction Enzyme Mix Adaptor-A (10 μM) Adaptor-B (10 μM) RNA Digestion Buffer RNA Digestion Enzyme MQ Binding Beads 2X HiFi PCR Master Mix Primer U (10 μM) Primer I (10 μM) Elution Buffer