Akt monoclonal antibody (5C10)

Product Specifications

Product Name Alternative

Protein kinase B, PKB

Certification

RUO

UniProt

P31749 (Akt1), P31751 (Akt2), Q9Y243 (Akt3)

Host

Mouse

Species Reactivity

Dog,Human,Mouse,Rat

Immunogen

Synthetic peptide corresponding to C-terminal human Akt (protein kinase B; PKB) conjugated to KLH.

Clone

5C10

Applications

ELISA, WB

Purification

Thiophilic adsorption and size exclusion chromatography purified.

Positive Control

Included. (Prod. No. 840-030-1)

Stability

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Dilution

Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Technical Note : There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg) . Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms. Thus the phosphorylation site Ser 473 of Akt1 corresponds to Ser 474 in Akt2 and Ser 472 in Akt3 respectively. Akt1: V 461 DSERRPHFPQFS 473 YSASSTA 480 UniProt ID P31749 Akt2: L 461 LELDQRTHFPQFS 474 YSASIRE 481 UniProt ID P31751 Akt3: D 461 NERRPHFPQFS 472 YSASGRE 479 UniProt ID Q9Y243 Replacement for ADI-905-661