ADAM17 (catalytic domain) (human), (recombinant) (His-tag)
Product Specifications
Specifications
≥1800 U/μg enzyme. One unit will hydrolyze one pmole Mca-PLAQAV-Dpa-RSSSR-NH 2 substrate (Prod. No. BML-P132) (10μM) per minute at 37°C, in 25mM TRIS, pH 9.0.
Product Name Alternative
TACE, A disintegrin and metalloproteinase 17, Tumor necrosis factor-α-converting enzyme
Certification
RUO
UniProt
P78536
Source
Produced in insect cells. Produced in a baculovirus expression system.
Sequence
Recombinant glycosylated catalytic domain (aa Pro 18 -Val 477) of ADAM17/TACE (A disintegrin and metalloproteinase 17; Tumor necrosis factor-α-converting enzyme), cloned from human cDNA (NM_003183), secreted as mature, active enzyme from insect cells, and purified using a C-terminal His-tag.
Purification
Purified by multi-step chromatography.
Stability
Salts (sodium chloride, calcium chloride, etc.) in the assay are inhibitory. ADAM17/TACE is stable after 6 freeze-thaws at ~0.4μg/μl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.
Purity
≥90% (SDS-PAGE)
Molecular Weight
~30.5kDa (calculated), ~36kDa doublet (SDS-PAGE)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 22.5mM TRIS, pH 7.5, containing 4.5μM ZnCl 2 , 0.0045% Brij-35 and 10% glycerol.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates, screen inhibitors.
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