Abl1 (mouse), (recombinant) (His-tag)
Product Specifications
Specifications
820 nmol/min/mg using a peptide substrate, EAIYAAPFAKKK, in 25mM MOPS, pH 7.2, containing 12.5mM β-glycerol-phosphate, 25mM MgCl 2 , 5mM EGTA, 2mM EDTA. Add 0.25mM DTT to assay buffer prior to use.
Product Name Alternative
Abelson murine leukemia viral oncogene homolog 1
Certification
RUO
UniProt
P00520
Source
Produced in insect cells. Produced in a baculovirus expression system.
Sequence
Mouse recombinant Abl1 (aa 27-1123) with N-terminal histidine tag. Mouse Abl1 is 89% identical (91% similar) to human Abl1.
Concentration
0.1 mg/mL
Purity
≥70% (SDS-PAGE)
Molecular Weight
~135kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Applications Notes
Useful tool to study enzyme regulation and kinetics, phosphorylate target substrates, screen for inhibitors.
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