[pThr286]Ca2+/calmodulin-dependent protein kinase II (rat) (α subunit) monoclonal antibody (22B1)

Synthetic peptide corresponding to aa 281-294 (M281HRQET (PSO3) VDCLKKFN294) of the α-subunit of mouse and rat CaM kinase II (Ca2+/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr286) .

Flow Cytometry (FACS, 1μg 106 cells) Immunocytochemistry (1:100) Immunofluorescence (1:100) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Recognizes the phosphorylated α-subunit of CaM kinase II. This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state.