[pSer473]Akt monoclonal antibody (11E6)

Flow Cytometry (intracellular staining, 2μg/ml) Immunohistochemistry (a starting dilution of 5μg/ml is recommended) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Technical Note : There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg) . Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms. Thus the phosphorylation site Ser 473 of Akt1 corresponds to Ser 474 in Akt2 and Ser 472 in Akt3 respectively. Akt1: V 461 DSERRPHFPQFS 473 YSASSTA 480 UniProt ID P31749 Akt2: L 461 LELDQRTHFPQFS 474 YSASIRE 481 UniProt ID P31751 Akt3: D 461 NERRPHFPQFS 472 YSASGRE 479 UniProt ID Q9Y243 Replacement for ADI-905-658