[pSer239]VASP monoclonal antibody (16C2)

Product Specifications

Product Name Alternative

Vasodilator stimulated phosphoprotein

Certification

RUO

UniProt

P50552

Host

Mouse

Species Reactivity

Human,Mouse,Rabbit,Rat

Immunogen

Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.

Clone

16C2

Applications

ELISA, Flow Cytometry, ICC, IP, WB

Purification

Thiophilic adsorption and size exclusion chromatography purified.

Stability

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Dilution

ELISA (use at 0.05μg/ml) Immunocytochemistry (use at 1-10μg/ml; may tolerate 0.5% formaldehyde fixation), Immunoprecipitation (use 1-10 μg per 106 pervanadate-treated A431 cells) Western Blot (1μg/ml for AP/BCIP/NBT (MTT) detection; 0.5μg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Important! The use of MAb to VASP (activated) (Ser 239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!