What is the catalog number for [C85A]UbcH5b (human), (recombinant) (His-tag)?

The catalog number for [C85A]UbcH5b (human), (recombinant) (His-tag) is BML-UW9065-0100.

What sizes are available for [C85A]UbcH5b (human), (recombinant) (His-tag)?

[C85A]UbcH5b (human), (recombinant) (His-tag) is available in the following size(s): 100 µg.

Is [C85A]UbcH5b (human), (recombinant) (His-tag) in stock?

[C85A]UbcH5b (human), (recombinant) (His-tag) is currently made to order / not held in immediate stock. Contact us for lead time.

Are there any special shipping requirements for [C85A]UbcH5b (human), (recombinant) (His-tag)?

[C85A]UbcH5b (human), (recombinant) (His-tag) is requires dry ice shipping.

[C85A]UbcH5b (human), (recombinant) (His-tag)

Product Specifications

Certification

RUO

UniProt

P62837

Source

Produced in E. coli.

Stability

Enzyme is stable to multiple freeze/thaw cycles.

Activity

The His 6 -tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His 6 -tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C 85 A] mutation completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control.

Molecular Weight

~18kDa

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 20mM TRIS-HCl, pH7.5, containing 0.5mM DTT.

Applications Notes

Negative control for ubiquitin conjugating enzyme UbcH5b (Prod. No. BML-UW9060) . The [C85A] mutation completely abolishes the ability of the enzyme to form a thiol ester.