8-pCPT-2′-O-Me-cAMP-AM

8-pCPT-2’-O-Me-cAMP-AM is rather labile in aqueous solution. Therefore, aqueous solutions should be freshly prepared immediately before use. Stock solutions in anhydrous DMSO or DMF should be relatively stable when stored frozen at -20°C to -80°C.

Due to its rather high lipophilicity, the solubility of 8-pCPT-2’-O-Me-cAMP-AM in water or buffers is limited. We suggest to use a small amount of anhydrous organic solvent such as anhydrous DMSO or DMF for preparation of 1-100mM stock solutions, and to dilute with water or buffer down to the concentrations required. In some cases, especially at high concentrations (~ 1mM), Pluronic® F-127 (Molecular Probes) can be useful to facilitate solubilization in water or physiological media. Please keep in mind that due to the high potency of 8-pCPT-2’-O-Me-cAMP-AM relatively low concentrations (1nM – 100μM) should be sufficient, and be sure to check for DMSO/DMF tolerance in your system. Since 8-pCPT-2’-O-Me-cAMP-AM is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise, serum esterases may strongly reduce the cell-loading efficacy. Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.