8-Br-2′-O-Me-cAMP-AM

8-Br-2′-O-Me-cAMP-AM is slowly hydrolized in water or buffer to yield 8-Br-2′-O-Me-cAMP (approx. 15%/day at RT) . Therefore solutions should be only freshly prepared immediately before use. Stock solutions in dry DMSO should be relatively stable when stored refrigerated.

8-Br-2’-O-Me-cAMP-AM is rather insoluble in water or buffers (<1mM) . For a stock solution we suggest to dissolve in a small volume of dry dimethylsulfoxide (DMSO) and to dilute aliquots with water up to the concentration needed. Please keep in mind that due to the high potency of this reagent relatively low concentrations (0.005-0.1mM) should be sufficient, and be sure to check for DMSO/DMF tolerance in your system. Since 8-Br-2’-O-Me-cAMP-AM is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise serum esterases may strongly reduce the cell-loading efficacy.Please rinse tube walls (and cap if necessary) carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.Please do not use ethanol or other potentially reactive solvents.