5′-Nucleotidase (from Crotalus atrox venom)

As indicated on product label or CoA when stored as recommended. Stable for at least 6 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing) . Frozen storage of smaller aliquots is recommended if more than 6 freeze-thaws of the entire vial contents (1000μl) are anticipated. Thawed enzyme is stable on ice for at least 2 hrs.

5’-nucleotidase is useful as the second enzyme in coupled assays of phosphodiesterases (3’,5’-cyclic nucleotide phosphodiesterase; EC 3.1.4.17) . Phosphodiesterase catalyzed hydrolysis of, for example, cAMP to 5’-AMP is coupled to 5’-nucleotidase cleavage of 5’-AMP to inorganic phosphate and adenosine. Phosphodiesterase activity is then quantified by colorimetric detection of the inorganic phosphate with a reagent such as BIOMOL Green (Prod. No. BML-AK111; see also Cyclic Nucleotide Phosphodiesterase Assay Kit, Prod. No. BML-AK800) .