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Recombinant Rhizobium meliloti Chitooligosaccharide deacetylase (nodB)

Product Specifications

Product Name Alternative

Nodulation protein B

Abbreviation

Recombinant Rhizobium meliloti nodB protein

Gene Name

NodB

UniProt

P02963

Expression Region

1-217aa

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti)

Target Sequence

MKHLDYIHEVPSNCDYGTEDRSIYLTFDDGPNPHCTPEILDVLAEYGVPATFFVIGTYAKSQPELIRRIVAEGHEVANHTMTHPDLSTCGPHEVEREIVEASEAIIAACPQAAVRHIRAPYGVWSEEALTRSASAGLTAIHWSADPRDWSRPGANAIVDAVLDSVRPGAIVLLHDGCPPDESGALTGLRDQTLMALSRIVPALHERGFAIRPLPPHH

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

30.6 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

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