Recombinant Human Asialoglycoprotein receptor 1 (ASGR1) -Detergent (Active)
Product Specifications
Product Name Alternative
Asialoglycoprotein receptor 1; ASGP-R 1; ASGPR 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1
Abbreviation
Recombinant Human ASGR1 protein-Detergent (Active)
Gene Name
ASGR1
UniProt
P07306
Expression Region
1-291aa
Organism
Homo sapiens (Human)
Target Sequence
MTKEYQDLQHLDNEESDHHQLRKGPPPPQPLLQRLCSGPRLLLLSLGLSLLLLVVVCVIGSQNSQLQEELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
Tag
C-terminal 10xHis-tagged
Type
MP-Detergent Transmembrane Protein & Active Protein & In Stock Protein
Source
Mammalian cell
Field of Research
Cancer
Relevance
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
1Measured by its binding ability in a functional ELISA. Immobilized Human ASGR1 at 2 μg/mL on an Nickel Coated plate can bind Anti-ASGR1 recombinant antibody (CSB-RA002207MA3HU) . The EC50 is 2.715-3.605 ng/mL. 2ASGR1 Recombinant Monoclonal Antibody (CSB-RA002207MA3HU) captured on Protein A Chip can bind Recombinant Human ASGR1 with an affinity constant of 0.716 nM as detected by MetaSPR Assay (WeSPRTM 200) .
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm sterile filtered 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, 6% Trehalose, pH 7.5
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
34.6 kDa
References & Citations
A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes. Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D. PLoS ONE 5:E12934-E12934 (2010)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Transmembrane Domains
1TM
Protein Length
Full Length
Available Sizes
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