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Recombinant Helicobacter pylori Prolipoprotein diacylglyceryl transferase (lgt)

Product Specifications

Abbreviation

Recombinant Helicobacter pylori lgt protein

Gene Name

Lgt

UniProt

O25609

Expression Region

1-284aa

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Target Sequence

MNAWNTIYDQFNPIAFSLGSIEVHWYGLAYACAIVTAFYMALRMIQKDPKRFPIERKEFESYFLWAELGIVLGARIGYILIYEPNSGYYLTHFWQIFNPFDSHGNFVGIRGMSYHGGLVGFLIASYLYSRKDLKKLLIYLDLIAISLPLGYVFGRIGNFLNQELVGRIVPKDSHLGQIIGIMVDNELRYPSQLIEAFLEGVIVFLMVMWAKKHTKTHGLLIVVYGLGYSLMRFIAEFYREPDSQMGVYFLNLSMGQILSLFMVIVSLGILLYATKNSKKIKENQ

Tag

N-terminal 10xHis-tagged

Type

CF Transmembrane Protein & Developed Protein

Source

In vitro E.coli expression system

Field of Research

Others

Relevance

Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.26.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

38.7 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Transmembrane Domains

7TM

Protein Length

Full Length

Available Sizes

Frequently Asked Questions

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