4E-BP1 (Phospho Thr37/46) (AB2302) Rabbit mAb

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1; eIF4E-binding protein 1; Phosphorylated heat-and acid-stable protein regulated by insulin 1; PHAS-I

WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

The antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr37and Thr46. The antibody does not cross-react with 4E-BP1 phosphorylated at other sites.