Src Family (Phospho Tyr416) (AB1618) Rabbit mAb

Product Specifications

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Synonyms

SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; ASV

Gene Name

SRC

Gene ID

6714

Swiss Prot

P07947; P07948; P06239; P08631; P12931; P06241

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Phospho Y416

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

The antibody detects endogenous levels of Src Family protein only when phosphorylated at Tyr416. The antibody does not cross-react with Src Family phosphorylated at other sites.