Chk1 (Phospho Ser345) (AB1614) Rabbit mAb

Product Specifications

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Synonyms

CHEK1, CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1

Gene Name

CHEK1

Gene ID

1111

Swiss Prot

O14757

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:500-1:2000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Phospho S345

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

The antibody detects endogenous levels of Chk1 protein only when phosphorylated at Ser345. The antibody does not cross-react with Chk1 phosphorylated at other sites.