Paxillin (Phospho Tyr118) (AB2185) Rabbit mAb

Product Specifications

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) . Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445) . [provided by RefSeq, Aug 2011]

Synonyms

PXN, Paxillin

Gene Name

PXN

Gene ID

5829

Swiss Prot

P49023

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Phospho Y118

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

The antibody detects endogenous levels of Paxillin protein only when phosphorylated at Tyr118. The antibody does not cross-react with Paxillin phosphorylated at other sites.