Histone H3 (Acetyl Lys9) (AB2087) Rabbit mAb

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21. 3. [provided by RefSeq, Aug 2015]

Synonyms

HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3. 1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3

Gene Name

HIST1H3A

Gene ID

8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968

Swiss Prot

P68431

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IHC, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:2000-1:10000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Acetyl Lys9

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

The antibody detects endogenous levels of Histone H3 protein only when acetylated at Lys9. The antibody does not cross-react with Histone H3 acetylated at other sites.