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Histone H2B (AB1923) Rabbit mAb

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21. 3. [provided by RefSeq, Aug 2015]

Synonyms

Histone H2B type 1-C/E/F/G/I (Histone H2B. 1 A, Histone H2B. a, H2B/a, Histone H2B. g, H2B/g, Histone H2B. h, H2B/h, Histone H2B. k, H2B/k, Histone H2B. l, H2B/l)

Gene Name

HIST1H2BC H2BFL, HIST1H2BE H2BFH, HIST1H2BF H2BFG, HIST1H2BG H2BFA, HIST1H2BI H2BFK

Gene ID

3017; 3017; 8345; 85236; 8340; 8342; 8341; 440689

Swiss Prot

P62807; P58876; Q93079; O60814; Q99880; Q99879; Q99877; Q5QNW6; P57053

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IHC, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:20000-1:50000 | IHC, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Unmodified

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

This antibody detects endogenous levels of Histone H2B protein.

Formulation

The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% Proclin 300.

Protein Name

Histone H2B

Available Sizes

Frequently Asked Questions

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