Cyclin B1 (phospho Ser126) (AB1665) Rabbit mAb

Product Specifications

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

Synonyms

CCNB1, CCNB, G2/mitotic-specific cyclin-B1

Gene Name

>>FoxO signaling pathway, >>Cell cycle, >>Oocyte meiosis, >>p53 signaling pathway, >>Cellular senescence, >>Progesterone-mediated oocyte maturation, >>Human immunodeficiency virus 1 infection

Gene ID

891

Swiss Prot

P14635

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant protein (or fragment) . This information is considered to be commercially sensitive.

Clonality

Monoclonal

Source

Monoclonal, Rabbit, IgG

Applications

WB, IHC, IF, IP, ELISA

Purification

The antibody was purified by protein A affinity chromatography.

Dilution

WB, 1:1000-1:5000 | IHC, 1:500-1:2000 | IF, 1:200-1:1000 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Modification

Phospho S126

Storage Conditions

Store at-20°C. Avoid freeze / thaw cycles.

Specificity

The antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at Ser126. The antibody does not cross-react with Cyclin B1 phosphorylated at other sites.