DDDDK-Tag (Flag-tag) (AB1623) Rabbit mAb
Product Specifications
Background
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Synonyms
Flag tag
Host
Rabbit
Immunogen
A synthetic peptide corresponding to DDDDK tag.
Clonality
Monoclonal
Source
Monoclonal, Rabbit, IgG
Applications
WB; IF; IP; ELISA
Purification
The antibody was purified by protein A affinity chromatography.
Dilution
WB, 1:1000-5000 | IF, 1:100-1:300 | IP, 0. 5μg-4μg antibody for 200μg-400μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modification
Unmodified
Storage Conditions
Store at-20°C. Avoid freeze / thaw cycles.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% Proclin 300.
Protein Name
Flag tag
Available Sizes
Documents
Frequently Asked Questions
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