Anti-FBG4 Antibody

Rabbit Polyclonal Antibody specific to FBG4 (F Box 4)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

F-box only protein 26

Gene Name

FBXO17

NCBI Gene ID

UniProt

Q96EF6

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids 70-85 (LQLARDRSAEGRALYA) of human FBG4 conjugated to KLH at the N- terminus.

Target Antigen

F-box only protein 17

Target

FBG4

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. Also recognizes sulfated glycans. Does not bind high-mannose glycoproteins.

Additionnal Information

Immunoblotting : use at 1:500 dilution. A band of approximately 31 kDa is detected. <br><br>Positive control: recombinant human full length FBG4 protein.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes amino acids human FBG4. Sequence homology to mouse and rat FBG4 protein is 100%.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

F-box proteins, critical components of the conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F- box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.