Mouse FOLR1 Protein, His Tag (Folic Acid Free)

Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD) . NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.2 kDa. The protein migrates as 32-34 kDa and 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P35846-1

Expression Region

Thr 25 - Ser 232

Host

HEK293

Target

FOLR1

Conjugation

Unconjugated

Tag

C-10xHis

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Folic acid-BSA conjugate at 1 μg/mL (100 μL/well) can bind Mouse FOLR1, His Tag (Cat. No. FO1-M5225) with a linear range of 0.3-10 ng/mL (QC tested).