DiSC3 (5)

DiSC3 (5) is a fluorescent probe commonly used as a tracer dye to evaluate mitochondrial membrane potential. The excitation/emission wavelength of DiSC3 (5) is up to 622/670 nm. DiSC3 (5) can inhibit the respiratory system associated with mitochondrial NAD, and the IC50 value is 8 μM. DiSC3 (5) in the presence of Na+/K+-ATPase inhibitor ouabain 2 can induce membrane hyperpolarization of Ehrlich ascites tumor cells[1][2][3].

Product Specifications

CAS Number

[53213-94-8]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/disc3-5.html

Purity

99.83

Solubility

DMSO : 5.83 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CCCN1/C(SC2=CC=CC=C12)=C\C=C\C=C\C3=[N+](CCC)C4=CC=CC=C4S3.[I-]

Molecular Formula

C25H27IN2S2

Molecular Weight

546.53

Precautions

H315, H319, H335

References & Citations

[1]Yamamoto T, et al. Multiple effects of DiS-C3 (5) on mitochondrial structure and function. Eur J Biochem. 2004;271 (17) :3573-3579. |[2]Sims PJ, et al. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry. 1974 Jul 30;13 (16) :3315-30.|[3]Smith TC, et al. The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells. Biochem Biophys Res Commun. 1980 Jul 31;95 (2) :722-7.