Biotinylated Human PD-L1 / B7-H1 Protein, Mouse IgG2a Fc, Avitag™ (MALS verified)

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1, which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 53.8 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_054862.1

Expression Region

Phe 19 - Arg 238

Host

HEK293

Target

PD-L1

Conjugation

Biotin-labeled

Tag

C-mFc (mIgG2a) & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Mouse IgG2a Fc,Avitag (Cat. No. PD1-H82A3) with a linear range of 5-39 ng/mL (QC tested).