Anti-Hsp90 Antibody

Mouse Monoclonal Antibody specific to Hsp90

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.6.4.10, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38

Gene Name

HSP90AA1

Gene ID

3320

NCBI Gene ID

UniProt

P07900

Accession Number

NP_001017963.2

Cellular Locus

Nucleus, Cytoplasm, Melanosome, Cell membrane, Mitochondrion

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human Hsp90 purified from E. coli.

Target Antigen

Heat shock protein HSP 90-α

Target

Hsp90

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ELISA, IHC, IP

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate rPubMed:11274138, PubMed:11276205, PubMed:12526792, PubMed:15577939, PubMed:15937123, PubMed:20628368, PubMed:24613385, PubMed:25609812, PubMed:27353360, PubMed:29127155, PubMed:25973397, PubMed:26991466, PubMed:27295069}.

Additionnal Information

Immunoblotting: use at 0.5-1ug/ml. A band of ~90 kDa is detected <br><br>User should determine optimal concentrations for their application. <br><br>Positive control: Heat-shocked HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Hsp90alpha and b. Other species have not been tested.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Hsp90 is an abundantly and ubiquitously expressed heat shock protein found in all eukaryotic cells. It exists in two principal forms, alpha and beta, which share 85% sequence homology. Both forms are expressed in the cytosol. Hsp90alpha exists primarily as a homodimer while Hsp90beta exists mainly as a monomer. Hsp90 plays a role in folding, assembly, maturation, and stabilization of specific proteins as a key component of a chaperone complex. Most of the Hsp90-regulated proteins that have been identified are involved in cell signaling; kinases, v-Src, Wee1, c-Raf, and p53 are some examples. When bound to ATP, Hsp90 interacts with co- chaperones Cdc37, p23, and various immunophilin-like proteins to form complexes that stabilize and protect target proteins from proteasomal degradation.