Resazurin (sodium)

Resazurin sodium (Diazoresorcinol sodium) is a non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent) . Resazurin sodium is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin sodium can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal) [1][2][3].

Product Specifications

CAS Number

[62758-13-8]

Product Name Alternative

Diazoresorcinol (sodium)

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Bacterial; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Anti-infection; Others

Field of Research

Infection

Assay Protocol

https://www.medchemexpress.com/Resazurin_sodium_salt.html

Purity

98.05

Solubility

DMSO : 2 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

O=C1C=CC2=[N+]([O-])C3=C(C=C(O[Na])C=C3)OC2=C1

Molecular Formula

C12H6NNaO4

Molecular Weight

251.17

Precautions

H315, H319, H335

References & Citations

[1]Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.|[2]Silva FSG, et al. Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay. Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15.|[3]Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel) . 2012;12 (9) :12347-60.