Anti-TrpC7 Ca+2 Channel Antibody

Mouse Monoclonal Antibody specific to TrpC7 Ca +2 Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

TrpC7, Transient receptor protein 7, TRP-7, hTRP7

Gene Name

TRPC7

NCBI Gene ID

UniProt

Q9HCX4

Accession Number

NP_065122.1

Cellular Locus

Cell membrane, Nucleus envelope

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 845-862 (FGNLNKDHLRVNKGKDI) of human TrpC7 (accession no. Q9HCX4). This sequence is 100

Target Antigen

Short transient receptor potential channel 7

Target

TrpC7 Ca+2 Channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion. {ECO:0000250}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~100kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat TrpC7.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Transient receptor potential cation channel, subfamily C, member 7 (TrpC7) has non-selective cation constitutive activity and susceptibility to negative regulation by extracellular Ca+2. TrpC7 plays an important role in the Ca+2 signaling pathway. TrpC7 is abundantly expressed in heart and may contribute to heart failure.