Anti-Arginase-1 Antibody

Mouse Monoclonal Antibody specific to Arginase-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.5.3.1, Liver-type arginase, Type I arginase

Gene Name

ARG1

NCBI Gene ID

UniProt

P05089

Cellular Locus

Cytoplasm, Cytoplasmic granule

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Arginase-1

Target Antigen

Arginase-1

Target

Arginase-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. {UniProtKB:Q61176, PubMed:15546957, PubMed:16709924, PubMed:19380772}.

Additionnal Information

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Human Arginase-1. Reactivity with other species has not been investigated.

Formulation

Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.

Buffer pH

pH 7.3-7.7

Target Background

The product of the ARG1 gene, Arginase-1, is a cytosolic metalloenzyme expressed predominantly in hepatocytes and plays an important role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the bloodstream. Arginase-1 has been identified as a specific marker of benign and malignant hepatic tumors.