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Anti-Osteonectin/SPARC Antibody

Mouse Monoclonal Antibody specific to Osteonectin (SPARC)

Product Specifications

Product Name Alternative

Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine

Gene Name

SPARC

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=SPARC">SPARC</a>

UniProt

P09486

Cellular Locus

Secreted, extracellular space, extracellular matrix, basement membrane

Host

Mouse

Reactivity

Human, Rabbit, Bovine, Porcine

Immunogen

Bovine bone osteonectin (SPARC)

Target Antigen

SPARC

Target

Osteonectin/SPARC

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, FACS

Field of Research

Cell adhesion

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Additionnal Information

ELISA: 1-10ug/ml with immobilized antigen <br><br>Western Blot: 1-10ug/ml, reducing and non-reducing conditions. <br><br>Immunohistochemistry: 1-10ug/ml, paraffin-embedded and frozen tissue sections.

Storage Conditions

This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/58008.pdf

Specificity

This antibody reacts with human, bovine, rabbit, and porcine bone-derived and platelet derived osteonectin as well as with thrombin-stimulated platelets.

Formulation

10 mM PBS, pH 7.4, 1% BSA, lyophilized

Buffer pH

pH 7.4

CAS Number

9007-83-4

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