Anti-GFRα-2 Antibody

Rabbit Polyclonal Antibody specific to GFR-2 (IN)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GDNF receptor α-2, GDNFR-α-2, GFR-α-2, GDNF receptor beta, GDNFR-beta, Neurturin receptor α, NRTNR-α, NTNR-α, RET ligand 2, TGF-beta-related neurotrophic factor receptor 2

Gene Name

GFRA2

Gene ID

2675

NCBI Gene ID

UniProt

O00451

Accession Number

NP_001158510.1

Cellular Locus

Cell membrane, Lipid-anchor, GPI-anchor

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 377- 391 of human GFRa-2. This peptide sequence is identical to those of mouse and rat.

Target Antigen

GDNF family receptor α-2

Target

GFRα-2

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.; [Isoform 2]: Participates in NRTN-induced 'Ser-727' phosphorylation of STAT3. {UniProtKB:O08842}.

Additionnal Information

Immunoblotting: use at 1:1,000-1:2,000 dilution. <br><br>Positive control: Whole cell lysate of HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat GFRalpha-2 (52 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuronal survivial and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphatidylinositol linked protein, NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned. NTNRalpha has also been referred to as GFRalpha-2, GDNFRbeta, Ret ligand 2 (RETL2), and TGF-beta-related neurotrophic factor receptor 2 (TmR2). GFRalpha-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRalpha-2, and the Ret PTK form a complex to transmit the NTN signal and to mediate NTN function.