Anti-CIDE-3 Antibody

Rabbit Polyclonal Antibody specific to CIDE-3

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Cell death-inducing DFFA-like effector protein C, Fat-specific protein FSP27 homolog

Gene Name

CIDEC

Gene ID

63924

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CIDEC">CIDEC</a>

UniProt

Q96AQ7

Accession Number

NP_001308071

Cellular Locus

Nucleus, Endoplasmic reticulum. Lipid droplet. Note=Diffuses quickly on lipid droplet surface, but becomes trapped and clustered at lipid droplet contact sites, thereby enabling its rapid enrichment at lipid droplet contact sites.

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids 10-25 (LLYPKSLSRHVSVRTS) of human CIDE-3 conjugated to KLH at the C- terminus.

Target Antigen

Cell death activator CIDE-3

Target

CIDE-3

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression. {PubMed:12429024, PubMed:18334488, PubMed:19843876, PubMed:20049731, PubMed:23399566}.

Additionnal Information

Immunoblotting: use at 1:500 dilution. A band of approximately 27 kDa is detected. <br><br>Positive control: HepG2 cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human CIDE-3. Sequence homology to the mouse CIDE-3 homolog, FSP27, is 93%.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

DNA fragmentation, induced by DNA fragmentation factor (DFF), is one of the critical steps in apoptosis. DFF is composed of two subunits, a 40-kDa caspase-activated nuclease (DFF40) and a 45-kDa inhibitor (DFF45). Recently a novel family of cell death- inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel CIDE. Two transcripts, CIDE-3 and CIDE-3alpha, are present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.