Recombinant Conus kinoshitai Mu-conotoxin KIIIB, partial

Mu-conotoxin KIIIA-P1: mu-conotoxins block voltage-gated sodium channels (Nav) . This toxin potently blocks Nav1.2/SCN2A (IC (50) 5-124 nM), Nav1.4/SCN4A (IC (50) =20-90 nM), and Nav1.7/SCN9A (IC (50) =290-413 nM) . It moderately blocks Nav1.1/SCN1A, and mNav1.6/SCN8A. It also shows a very low activity on Nav1.3/SCN3A. This toxin binds a microsite within the pore different from the tetrodotoxin binding site 1 (tested on Nav1.2) . The block is partial, with a residual current that can be completely blocked by TTX. The toxin probably docks at a more superficial site in the outer vestibule of the channel than does TTX. On rNav1.2/SCN2A, it produces a block that is only partially reversible. The block of Nav1.7 is modified when beta-subunits are coexpressed with the alpha subunit. Hence, blocks of channels containing beta-1 and beta-3 subunits are more potent (compared to channels without beta subunits), whereas blocks of channels containing beta-2 and beta-4 subunits are less potent (compared to channels without beta subunits) . ; Mu-conotoxin KIIIA-P2: This toxin potently blocks Nav1.2/SCN2A (Kd=230 nM, IC (50) =1.37 uM) and Nav1.4/SCN4A (Kd=830 nM, IC (50) =2 uM) . It also moderately blocks Nav1.7/SCN9A (Kd=1.57 uM, IC (50) =5.4 uM) . In addition, this toxin may also inhibit other sodium channels, as does Mu-conotoxin KIIIA-P1. ; Mu-conotoxin KIIIA-N: This toxin moderately blocks Nav1.2/SCN2A (IC (50) =875 nM), Nav1.4/SCN4A (IC (50) =472 nM), and Nav1.7/SCN9A (IC (50) =887 nM) . ; Mu-conotoxin KIIIB-P1: This toxin potently blocks Nav1.2/SCN2A (Kd=470 nM) . In addition, this toxin may also inhibit other sodium channels, as does Mu-conotoxin KIIIA-P1. ; Mu-conotoxin KIIIB-P2: This toxin potently blocks Nav1.2/SCN2A (Kd=26 nM) . In addition, this toxin may also inhibit other sodium channels, as does Mu-conotoxin KIIIA-P1.

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.