What is the catalog number for Phosphate Buffer, 10X?

The catalog number for Phosphate Buffer, 10X is AR-6565-05.

What sizes are available for Phosphate Buffer, 10X?

Phosphate Buffer, 10X is available in the following size(s): 1000 mL.

Is Phosphate Buffer, 10X in stock?

Phosphate Buffer, 10X is currently made to order / not held in immediate stock. Contact us for lead time.

Phosphate Buffer, 10X

This buffer is supplied as 10X solution. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc.

Product Specifications

Applications

WB, IHC, ELISA

Assay Principle

Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8oC for several weeks. Application: Wash membrane or ELISA plates for 10 minutes each for three times.. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.