Azithromycin ELISA Kit

Azithromycin is a broad-spectrum antimicrobial drug that is used to treat respiratory, enteric, and genitourinary infections. It targets both gram-positive and gram-negative bacteria. The mode of action of this drug is that it binds to the 23S rRNA of the 50S ribosomal subunit of the bacteria. This prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. Antibiotics such as Azithromycin have been used in food animals and aquaculture either to treat illnesses or as growth promoters. However, the use of Azithromycin in food animals may result in the formation of resistant bacteria and may lead to antibiotic resistance in humans. BioVision’s Azithromycin ELISA kit is used to quantitatively measure Azithromycin in tissues, honey, and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Azithromycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Azithromycin in the samples.