Human IL-37 Protein, His tag (Animal-Free)

Human IL-37 Protein, His tag (Animal-Free), expressed in E. coli

Product Specifications

Background

Humaninterleukin-1 family member 7 (IL-37) is a member of interleukin-1 cytokine family. It is reported that five alternative splicing transcript variants encoding different subtypes have different expression profiles. The longest IL-37 transcript is called IL-37b or IL-37 isoform 1, which encodes a propeptide with 218 amino acid residues and a propeptide with 45 amino acids, and is cleaved to produce a mature protein. IL37b binds to IL18 Rα with low affinity, but does not produce any IL-18 excitation or antagonism

Product Name Alternative

IL-1F7, IL-1ζ (zeta), IL-1H4, FIL1, FIL1Z

Accession Number

Q9NZH6

Tag

IL-37

Type

Cytokines and proteins

Applications

His tag

Purity

> 95% as determined by SDS-PAGE.

Activity

Measure by its ability to induce IL-8 secretion in human PBMCs.The ED₅₀ for this effect is <0.9 μg/mL.

Molecular Weight

18.7 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

The product is shipped with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human IL-37 Protein consists of 166 amino acids and predicts a molecular mass of 18.7 kDa

Formulation

The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.

Applications Notes

Human

Other Product Names

Human IL-37 Protein, His tag (Animal-Free), IL-37, IL-1F7, IL-1ζ (zeta), IL-1H4, FIL1, FIL1Z

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously